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Large-fragment deletion in <t>E.</t> <t>coli</t> MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.
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e coli strain jm109  (Zymo Research)
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Large-fragment deletion in <t>E.</t> <t>coli</t> MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.
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Large-fragment deletion in <t>E.</t> <t>coli</t> MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.
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jm109 e coli strains  (Addgene inc)
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Large-fragment deletion in <t>E.</t> <t>coli</t> MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.
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e. coli strain jm109(de3)  (Promega)
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Large-fragment deletion in <t>E.</t> <t>coli</t> MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.
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e. coli overexpression strain jm109 (de3)  (Promega)
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Large-fragment deletion in <t>E.</t> <t>coli</t> MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.
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Large-fragment deletion in E. coli MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.

Journal: Applied and Environmental Microbiology

Article Title: A recombineering-based platform for high-throughput genomic editing in Escherichia coli

doi: 10.1128/aem.00193-25

Figure Lengend Snippet: Large-fragment deletion in E. coli MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.

Article Snippet: E. coli strains JM109 (ATCC 53323), MG1655 (ATCC 47076), JW128 , Nissle 1917 , and DH10B served as genetic materials in genomic editing experiments.

Techniques: Sequencing